Gefocusseerde ultrasone apparaten BoFU-80
MALDI-TOF can accurately identify bacteria, fungi, etc. based on the specific mass-to-charge ratio (m/z) of microorganisms. However, difficult-to-break microorganisms such as mycobacteria and filamentous fungi limit their detection rate. A large number of bacterial cells are required and the steps such as ethanol inactivation and formic acid-acetonitrile wall-breaking are required to extract proteins for detection.
BoFU-80 Focused Ultrasonicators Features
- Convenient and fast: Add the bacterial colony to the sample tube containing the extractant to perform ultrasound, and the entire operation takes less than 5 minutes.
- Safe and pollution-free: Independently sealed sample tubes, no need to open the cap repeatedly, reducing operating steps and reducing the probability of contamination of operators and the experimental environment.
- Small sample volume required: Only 1-2 colonies (mycobacteria, Nocardia, etc.) or a small number of spores can be used to obtain sufficient protein for detection.
- Improving the detection rate: Ultrasound has a solubilizing effect, which allows the extractant to penetrate the cell wall faster and achieve a good crushing effect. More proteins can be obtained under the same bacterial content, which improves the detection rate.
Description of BoFU-80 Focused Ultrasonicators
MALDI-TOF can accurately identify bacteria, fungi, etc. based on the specific mass-to-charge ratio (m/z) of microorganisms. However, difficult-to-break microorganisms such as mycobacteria and filamentous fungi limit their detection rate. A large number of bacterial cells are required and the steps such as ethanol inactivation and formic acid-acetonitrile wall-breaking are required to extract proteins for detection.
BoFU-80 uses a high-frequency confocal ultrasound system to efficiently concentrate acoustic energy into the sample to be processed, greatly improving the strain breaking efficiency and effectively improving the protein extraction rate of the strain. It can effectively improve the efficiency of Merieux, Bruker and Autobio etc. Detection rate of MALDI-TOF system. For pre-treatment of mycobacteria and filamentous fungi that are difficult to break, the ultrasonic process can be completed in just 60 seconds, and the entire process takes less than 5 minutes. The ultrasonic process is carried out in a closed tube, without the need to repeatedly open the lid, reducing operating steps and avoiding pollute.
Applications of BoFU-80 Focused Ultrasonicators
- Cell Lysis
- Proteomics
- Chromatin
Why Choose Our Focused Ultrasonicator?
Longlight’s focused ultrasound sample meets the needs of nucleic acid and protein sample preparation by providing highly controllable and focused ultrasonic energy to the samples. It adopts a compact desktop design. With its advanced technology and precise performance, it brings cost advantages and service capabilities to the world, becoming an important tool for sample pre-processing in the fields of second-generation sequencing, proteomics, etc.
Advanced technology
Different from traditional water bath and probe ultrasound equipment, BoFU focused ultrasound system can concentrate all the ultrasound energy on the sample container. The ultrasound process is only carried out in an isothermal, non-contact water bath environment, without energy loss, bringing stable and reliable experimental results.
Simple sample handling
The sample is ultrasonically processed in a closed sample tube. Simply place the sample in the ultrasonic tube for ultrasonic processing. Various types of samples can be processed in a very short time. It is suitable for DNA/RNA and chromatin shearing, FFPE Extraction, Cell Lysis, Lysis of difficult-to-break microorganisms, Tissue Lysis and Extraction, etc.
Standardized experimental results
Each Focused Ultrasonicator is carefully calibrated by engineers to ensure uniform performance of all instruments. Highly sensitive temperature control system ensures that the sample is not affected by the heat generated during the ultrasonic process. It greatly reduces the error introduced by manual operation and provides standardized experimental results.
Neem contact met ons op
Online bericht
Voor een snellere reactie kunt u contact met ons opnemen via telefoon of WhatsApp: +86 13590383492.
